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101.
Proteins governing cell death form the basis of many normal processes and contribute to the pathogenesis of many diseases when dysregulated. Here we report the cloning of a novel human CED-4-like gene, CLAN, and several of its alternatively spliced isoforms. These caspase-associated recruitment domain (CARD)-containing proteins are expressed at varying degrees in normal human tissues and may contribute to a number of intracellular processes including apoptosis, cytokine processing, and NF-κB activation. The CARD of the CLAN proteins binds a number of other CARD-containing proteins including caspase-1, BCL10, NOD2, and NAC. Once their physiologic functions are uncovered, CLAN proteins may prove to be valuable therapeutic targets. 相似文献
102.
103.
David C. Nickle Gerald H. Learn Matthew W. Rain James I. Mullins John E. Mittler 《Journal of molecular evolution》2002,54(1):134-137
Studies of ancient DNA have attracted considerable attention in scientific journals and the popular press. Several of the
more extreme claims for ancient DNA have been questioned on biochemical grounds (i.e., DNA surviving longer than expected)
and evolutionary grounds (i.e., nucleotide substitution patterns not matching theoretical expectations for ancient DNA). A
recent letter to Nature from Vreeland et al. (2000), however, tops all others with respect to age and condition of the specimen. These researchers
extracted and cultured a bacterium from an inclusion body from what they claim is a 250 million-year (Myr)-old salt crystal.
If substantiated, this observation could fundamentally alter views about bacterial physiology, ecology and evolution. Here
we report on molecular evolutionary analyses of the 16S rDNA from this specimen. We find that 2-9-3 differs from a modern
halophile, Salibacillus marismortui, by just 3 unambiguous bp in 16S rDNA, versus the ∼59 bp that would be expected if these bacteria evolved at the same rate
as other bacteria. We show, using a Poisson distribution, that unless it can be shown that S. marismortui evolves 5 to 10 times more slowly than other bacteria for which 16S rDNA substitution rates have been established, Vreeland
et al.'s claim would be rejected at the 0.05 level. Also, a molecular clock test and a relative rates test fail to substantiate
Vreeland et al.'s claim that strain 2-9-3 is a 250-Myr-old bacterium. The report of Vreeland et al. thus falls into a long
series of suspect ancient DNA studies.
Received: 12 April 2001 / Accepted: 9 June 2001 相似文献
104.
John Williams 《BMJ (Clinical research ed.)》2002,325(7371):1016-1017
105.
The extant jawless fishes (Agnatha) include the hagfishes andlampreys whose ancestry can be traced through a conserved evolutionto the earliest of vertebrates. This review traces the studyof the enteropancreatic (EP), endocrine cells and their productsin hagfishes and lampreys over the past two centuries. ErikaPlisetskaya is one of several prominent comparative endocrinologistswho studied the development, distribution or function of theagnathan EP system. Her physiological studies in Russia laidthe foundation for her subsequent isolation in North Americaof the first lamprey EP peptides (insulin and somatostatin)and providing the first homologous radioimmunoassay for agnathan(lamprey) insulin. This review also emphasizes the nature andthe method of development of the agnathan endocrine pancreas(islet organ), for it reflects the earliest vertebrate endocrinepancreas originating from intestinal and/or bile-duct epithelia.The lamprey life cycle includes a protracted larval period anda metamorphosis when the adult EP system develops. Differencesin morphogenesis during metamorphosis of southern- and northern-hemispherelampreys dictate that a single cranial mass (islet organ) appearin the former and both a cranial and a caudal principal isletcomprises most of the islet organ in holarctic species. Thereare differences in distribution of cell types and in the primarystructure of the peptides in the definitive islet organ of hagfishesand lampreys. The primary structures of insulin, somatostatins,glucagons, glucagon-like peptide, and peptide tyrosine tyrosineare now available for three lamprey species representing threegenera and two of the three families. Differences in structureof peptides within, and between, families is providing supportfor earlier views on the time of divergence of the familiesand the different genera. It is concluded that due to the ancientlineage and successful habitation of lampreys and hagfishes,and the importance of the EP system to their survival, thattheir EP systems should be a research focus well into the nextcentury. 相似文献
106.
107.
Ancient landscapes, which have not been glaciated in recent times or disturbed by other major catastrophic events such as volcanic eruptions, are dominated by nutrient-impoverished soils. If these parts of the world have had a relatively stable climate, due to buffering by oceans, their floras tend to be very biodiverse. This review compares the functional ecophysiological plant traits that dominate in old, climatically buffered, infertile landscapes (OCBILS) with those commonly found in young, frequently disturbed, fertile landscapes (YODFELs). We show that, within the OCBILs of Western Australia, non-mycorrhizal species with specialised root clusters predominantly occur on the most phosphate-impoverished soils, where they co-occur with mycorrhizal species without such specialised root clusters. In global comparisons, we show that plants in OCBILs, especially in Western Australia, are characterised by very low leaf phosphorus (P) concentrations, very high N:P ratios, and very high LMA values (LMA = leaf mass per unit leaf area). In addition, we show that species in OCBILs are far more likely to show P-toxicity symptoms when exposed to slightly elevated soil P levels when compared with plants in YODFELs. In addition, some species in OCBILs exhibit a remarkable P-resorption proficiency, with some plants in Western Australia achieving leaf P concentrations in recently shed leaves that are lower than ever reported before. We discuss how this knowledge on functional traits can guide us towards sustainable management of ancient landscapes. 相似文献
108.
Edward John Tilt 《BMJ (Clinical research ed.)》1890,1(1533):1167-1168
109.
110.